IN VITRO CONSERVATION OF ORCHIS LATIFOLIA : A THREATENED, MEDICINAL TERRESTRIAL ORCHID

Abstract

In vitro shoot multiplication of Orchis latifolia, a threatened medicinal orchid has
been achieved on MS medium supplemented with BAP (1.0 mg/l) and NAA (0.1
mg/l) using :;prouting buds as explants. Occasionally "protocorm" like structures
were also produced on the same medium: The in vitro raisd shoots could be
successfully conserved for more than 10 months at lOoe without Intermittent
subcultl'~e.