Polymerase Chain Reaction for Evaluation! Detection of Transgenic Planting Material

Abstract

Transgenic crops developed using recombinant DNA technology can make a significant contribution to global food, feed and fibre security, and alleviation of poverty, particularly in the Third World (Dunwell, 1999). Global area under cultivation of transgenic crops has increased from 1.7 million hectares in 1996 to 81 million hectares in 2004 (James, 2004). The commonly used regulatory sequences to transform most ofGMplants are Cauliflower Mosaic Virus promoter (CaMV 35S) and the Agrobacterium tumefaciens nopaline synthase terminator. Consequently, methods detecting one of these regulatory sequences are popular for screening purposes.