Evaluation of Genetic Diversity in Indian Durum Wheat with RAPD Markers

Abstract

Genetic variability among 26 released varietiesllocal cultivars of Triticum durum was estimated by RAPD marker
analysis and the results discussed in view of their centre of breeding and the pedigree. A total of 4 series of primers
were used ,0 screen the polymorphic primers and the profiles generated by 15 of such types were used for analysis.
The primers OPA3 and OPP6 were the most polymorphic primers and can be used in conjugation with the
morphological markers for the identification of the varieties. The varieties developed by the selection among the
local race Bansi were grouped together. The highest degree of polymorphism was between the locallandrace Malvaraj
with Motia and Jay. The clustering on the basis of RAPD markers in the present study was clearly on the pattern
of their parentage. The varieties developed through the selection in the CIMMYT material were widely dispersed
and therefore can be used along with the locallandraces in the breeding programme to diversify the genetic base.
The grouping of the germplasm particularly the landraces by RAPD markers can be used in developing the Indian
durum wheat cultivars with wider genetic base.