Development of Genic Simple Sequence Repeat Markers as Novel Genomic Resources in Dolichos Bean (Lablab purpureus L.)
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Abstract
Dolichos bean (Lablab purpureus L.) is an old cultivated pulse of Africa, Asia and Australia. In spite of its
multiple uses, there are limited genomic resources available, which could facilitate genetic diversity studies
and DNA fi ngerprinting. Here, we report the extraction of SSR markers from the Expressed Sequence Tags
(ESTs) of dolichos bean. From a total 1129 unique ESTs of dolichos bean available in public domain, a total
of 83 SSRs were identifi ed. Among these EST-SSRs, tri-nucleotide repeats were most abundant (37) followed
by tetra-nucleotide repeats (18) and di-nucleotide repeats (15). Functionality of the novel SSRs were validated
by successful PCR amplifi cation in nine varieties of dolichos bean using ten SSR markers. The novel EST-SSR
markers are expected to be helpful in genetic diversity studies as well as for varietal DNA fi ngerprinting in
this important legume.