SSR Markers based Diversity Analysis in Elite Genotypes of Pearl Millet
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Abstract
Analysis of genetic diversity was carried out using 32 genotypes of pearl millet using SSR markers and phenotypic data of 10 morphological traits. Significant differences were observed for all the traits recorded in the study, indicating the availability of sufficient genetic variability in the materials. Among the 69 simple sequence repeat (SSR) primers screened, 44 primers were found polymorphic. These polymorphic primers amplified 151 alleles. The number of alleles per SSR marker varied from 2 to 5 per locus. Polymorphism information content values (PIC) ranged from 0.058 to 0.608 per locus with an average of 0.375. Genotypes were grouped into three clusters based on molecular and phenotypic data, and the genotypes’ distribution was similar in both. Principal coordinate analysis further corroborated the grouping of genotypes obtained on phenotypic and molecular data. Structure analysis was also observed to be in line with cluster grouping as well as principal coordinate analyses.
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