SDS-PAGE Seed Protein Profiles as Distinguishing Features in Certain Varieties of Field Crops

Abstract

A total of 74 released varieties of field crops (15 in wheat, 9 in maize, 19 in soybean, 6 in mung, 7 in md, 8 in lentil
and 10ingram)at Pantnagar, Uttarakhand were characterized usingSDS-PAGE(sodium dodccyl sulphate polyacrylamide
gel electrophoresis) of seed protein extracted in Tris-HCI buffer with the objective of finding certain critical bands
which could be used as distinguishable features. Protein profiles of different field crops showed the number of bands
ranging from 11 (urd, lentil and gram) to 16 (maize). Wheat and soybean displayed 12 bands each and mung showed
14 bands. It was possible to distinguish UP 2565 vs C 306, PBW 343, PBW 396, PBW 262, UP 1109, UP 2338. UP
2382 and UP 2425 vs Raj 3765, HD 2687, PBW 373, PBW 502 and UP 21 J3 vs PBW 175 in wheat; Pant Mung I and
Pant Mung 2 from Pant Mung 3, Pant Mung 4, Pant Mung 5 and UPM 0205 in mung; Pant U 30, Pant U 35 ancl UPU
0031 from Manikya, UPU 97-10, Narendra Urd-l and Pant U 19 in urcl; PL 4 and PL 406 from PL 02, PL 5, PL 018,
PL 023, PL 234 and PL 639 in lentil and PG 118 from PGK 23, PG K 24, PG 033, PG 034, PG 035, PG 036, PG 037,
PG 038 and PantG 114 in gram on the basis ofprotein profiles. SDS-PAGEcould not distinguish 19 varieties ofsoybean
and 9 varieties of maize. These critical bands could serve as additional descriptors in DUS testing.