SOMATIC EMBRYOGENESIS IN WALNUT (Juglans regta 1.)

Abstract

In Walnut (juglans regia L) cultivars ViZ., 'Netar Akhrot' and 'Govind', somatic embryos were
produced on embryonic axis and cotyledonary tissue explants cultured on DKW medium supplemented
with 2mg/1 kinetin, Img/l BA and 0.01 mg/l IBA for 3 weeks. In the present investigation,
apparendy normal bipolar somatic embryos have been obtained. However, since the explant source
is immature embryo, the culture represents varying genotypes. The difference could be seen from
differential response of explants to culture condition. Attempts were made to regenerate plants from
somatic embryos