MICROPROPAGATION OF Commiphora wightii (ARNOTT) BHANDARI A THREATENED MEDICINAL PlANT OF SEMI-ARID REGION

Abstract

A simple and efficient micropropagation method is developed for Commiphora wightii from the
cotyledonary nodal segments of twenty one-day-old in vitro grown seedlings. Multiple shoots were
initiated on MS medium containing IAA 0.1 mgl-1, BA 1.5 mgl-1 and additives consisting of 50
mgl-1 ascorbic acid, 25 mgl-1 each of citric acid, arginine and adenine sulfhate. The isolated shoots
were multiplied on MS medium containing IAA 0.1 mgr1
, BA 1.0 'mgr and additives as in the
initiation medium. Shoots were rooted on 1/2 strength MS medium containing 0.25 mgl-1 IBA.
The rooted plantlets were hardened and transferred to soil