Evaluation of Chilli Genotypes against Pepper Mottle Virus under Artificial Conditions

Abstract

One hundred and twenty four genotypes of Capsicum annuum were screened for resistance against Pepper mottle virus (PepMoV). Artificial screening was done via mechanical inoculation. Phenotypically resistant lines were further subjected against PepMoV antibodies in double antibody sandwich ELISA (DAS-ELISA). The results deciphered that seven genotypes viz., SM-478, IS-263, COO-226, VR-523, PL-412, IS-269, KC-312 exhibited resistant reaction (R), whereas twenty showed moderately resistant reaction. Virus distribution in hot pepper was seen via exposing different plant parts to DAS-ELISA. Maximum absorbance was recorded in leaf tissues. Further the effect of virus on seed yield was also observed in seven genotypes, which included two popular varieties viz., Punjab Tej and Suraj Mukhi and one hybrid CH-1. The results concluded that virus can cause up to 72 per cent seed yield loss in susceptible genotype i.e KRS-303.