OPTIMIZATION OF PCR CONDITIONS FOR GENETIC CHARACTERIZATION OF KENYAN BANANAS AND PLANTAINS (Musa ssp.)

Abstract

Optimization of the reaction conditions for Polymerase Chain Reaction (PCR) should be done before
the actual Random Amplified Polymorphic DNA (RAPD) analysis is carried out to get repeatable
results. Optimizations of concentration was carried out the reaction components; template DNA and
Taq DNA Polymerase. The optimum concentrations were determined by the ability to form
amplification products with the highest consistency. The significance of optimization is discussed and
the results compared with similar published ones.